cy3 conjugated antirabbit secondary antibody (Jackson Immuno)

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Jackson Immuno cy3 conjugated antirabbit secondary antibody
Cy3 Conjugated Antirabbit Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 94/100, based on 283 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/donkey+antirabbit+cy3+secondary+antibody/Cy+3+AffiniPure+F(ab')%E2%82%82+Fragment+Donkey+Anti-Rabbit+IgG/pm40394655-313-11-17
Average 94 stars, based on 283 article reviews

cy3 conjugated antirabbit secondary antibody - by Bioz Stars, 2026-09

94/100 stars

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Immunohistochemistry:

Article Title: Metabolic Regulation of Hippocampal Neuroprogenitor Apoptosis After Irradiation.
Article Snippet: Methods for TUNEL (In Situ Cell Death Detection Kit, Roche Diagnostics, Indianapolis, IN) were as previously described (4). .. A rabbit cleaved caspase-3 (1:1000, Cell Signaling Technology, Danvers, MA) antibody and donkey antirabbit Cy3 secondary antibody (1:200, Jackson ImmunoResearch, West Grove) were used for caspase-3 immunohistochemistry. .. DNA damage foci were assessed using an antibody against the phosphorylated histone variant (cH2AX, 1:1000, Millipore, Billerica, MA) (4).

Expressing:

Article Title: Taurine suppresses the spread of cell death in electrically coupled RPE cells
Article Snippet: Antibody binding was visualized by fluorescent microscopy using goat antirabbit Alexa 488 secondary antibody (Molecular Probes, Inc., Eugene, OR) as well as with a sheep antirabbit FITC secondary antibody (Jackson ImmunoResearch Laboratories, West Grove, PA). .. Cells were similarly examined for the expression of connexin 46, by incubating with a polyclonal rabbit anticonnexin 46 antibody (a gift from Dr. Nalin Kumar, Department of Ophthalmology, University of Illinois College of Medicine, Chicago, IL), and a donkey antirabbit cy3 secondary antibody (Jackson ImmunoResearch Laboratories). .. Differential interference contrast (DIC), phase contrast, and fluorescence images were collected using either a Zeiss Axiovert 100 M microscope (Zeiss, Oberkochen, Germany) or an Olympus Fluoview 300 confocal microscope.

Staining:

Article Title: Formation of perineuronal nets within a thalamocortical circuit shapes mechanical and thermal pain thresholds in mice with neuropathic pain
Article Snippet: Wisteria Floribunda Agglutinin fluorescent staining was performed using biotin-conjugate WFA (1:500; #L1516, Sigma-Aldrich, Darmstadt, Germany; RRID: AB_2620171) and fluorescent secondary antibody (Streptavidin Alexa Fluor 488, 1:200, Invitrogen, Carsband, CA). .. We also performed double immunofluorescent staining for WFA and PV (monoclonal rabbit anti-PV antibodies; 1:1000, #PV27, Swant, Switzerland; RRID: AB_2631173) using secondary Alexa Fluor 488 antibody (1:200, Jackson ImmunoResearch, Cambridge, United Kingdom) and donkey antirabbit Cy3 secondary antibody (1:200, Jackson ImmunoResearch, Cambridge, United Kingdom) and single labelling of MMP-9 (rabbit anti-MMP-9 antibody 1:100, #AB19016, EMD Millipore, Darmstadt, Germany). .. We also stained sections with the following primary antibodies: anti-Cat-315 detecting aggrecan core protein (mouse, 1:200, #MAB1581 EMD Millipore, Darmstadt, Germany, RRID:AB_11214066), antiaggrecan (rabbit, 1:200, #AB1031, Merck, Darmstadt, Germany, RRID:AB_90460), antisomatostatin (rabbit, #20067, 1:1000, Immunostar Hudson, Wisconsin 54,016, RRID:AB_572264), and anti-5HT 3A (rabbit, 1:100 for 48 hours, #NB100-56351, Novus Biologicals, CO 80112, RRID:AB_838865).

Article Title: Formation of perineuronal nets within a thalamocortical circuit shapes mechanical and thermal pain thresholds in mice with neuropathic pain.
Article Snippet: We moved from the hypothesis that perineuronal nets (PNNs), which are condensed structures of the extracellular matrix surrounding GABAergic interneurons in the forebrain, contribute to mechanisms of maladaptive neuronal plasticity underlying chronic pain.. Here, we found that the density of PNNs labelled with the lectinWisteria Floribunda Agglutinin (WFA) increased in the contralateral somatosensory cortex (SSC), medial prefrontal cortex (mPFC), reticular thalamic nucleus (RTN), and insular cortex of mice developing neuropathic pain in response to unilateral chronic constriction injury of the sciatic nerve.. These regions are involved in neuronal circuits underlying perception, sufferance, embodiment, and top-down control of pain.

Article Title: Revisiting hydrogen peroxide as radiosensitizer for solid tumor cells.
Article Snippet: Background and purpose: Tumor hypoxia is the principal cause of clinical radioresistance.. Despite its established role as radiosensitizer, hydrogen peroxide (H2O2) encounters clinical limitations due to stability and toxicity concerns.. Recent advancements in drug delivery combine H2O2 with sodium hyaluronate (SH), enabling intratumoral administration of H2O2.

Incubation:

Article Title: Upregulation of neurokinin-1 receptor expression in rat spinal cord by an N-terminal metabolite of substance P.
Article Snippet: Chronic in ̄ammatory conditions are associated with an upregulation of both substance P (SP) and neurokinin-1 (NK-1) receptors in the dorsal spinal cord.. These receptors have been implicated in hyperalgesia as well as stress-induced analgesia.. On the basis of the release of SP during chronic pain, and its rapid metabolism, we tested the hypothesis that SP metabolites regulate the synthesis of either SP or NK-1 receptors in the spinal cord.

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